The column with a 4.6mm ID may not be sufficient to obtain around 10 mg of product by LC-MS purification. The maximum mass load for this column size would typically be around 500ug.
1.50458
SeQuant® ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 250 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel
Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
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About This Item
UNSPSC Code:
41115709
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Product Name
SeQuant® ZIC-HILIC™ 5µm,200Å 250 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
500 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
25 cm × 4.6 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
1 of 4
This Item | 1.50457 | 1.50497 | 1.50481 |
|---|---|---|---|
| matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase | matrix active group ZIC-HILIC bonding phase |
| separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) | separation technique hydrophilic interaction (HILIC) |
| particle size 5 μm | particle size 5 μm | particle size 5 μm | particle size 5 μm |
| agency suitable for USP L114 | agency suitable for USP L114 | agency suitable for USP L114 | agency suitable for USP L114 |
| matrix fully porous particle | matrix fully porous particle | matrix fully porous particle | matrix fully porous particle |
| pore size 200 Å pore size | pore size 200 Å pore size | pore size 200 Å pore size | pore size 200 Å pore size |
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 4.6 mm ID is the best choice for the separation of complex samples using a standard HPLC Instrument.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Analysis Note
Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40
Symmetry (Cytosine): 0.95 - 1.40
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Can a column with a 4.6mm ID be used to obtain around 10 mg of product by LC-MS purification? Also, what is the maximum mass load for your column with a 4.6mm ID?
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