MilliporeSigma
All Photos(1)

1.52258

Supelco

Chromolith® WP 300 Protein A

L × I.D. 25 mm x 4.6 mm HPLC column

Synonym(s):
Chromolith® WP 300 Protein A 25-4.6 HPLC column

material

PEEK column

product line

Chromolith®

feature

endcapped: no
endcapped: no

packaging

1 ea of

parameter

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

column L × I.D.

2.5 cm × 4.6 mm

matrix

monolithic silica
monolithic silica

matrix active group

Protein A bonding phase
Protein A bonding phase

pore size

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

surface area

120 m2/g

operating pH range

1.5-7.5

operating pH

1.5-7.5

separation technique

affinity

storage temp.

2-8°C
2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service