16-157
Protein A Agarose/Salmon Sperm DNA, 2.5 mL
for use in chromatin immunoprecipitations (ChIP assays)
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Synonym(s):
ChIP agarose beads, ChIP agaraose A beads, ChIP assays
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32
Recommended Products
biological source
Staphylococcus aureus
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
suitability
suitable for immunoprecipitation
shipped in
wet ice
General description
Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
Application
Suitable for use in chromatin immunoprecipitation (ChIPs) assays
Quality
Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
Physical form
10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK 1
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