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Protein A Agarose/Salmon Sperm DNA, 2.5 mL

Name: Protein A Agarose/Salmon Sperm DNA, 2.5 mL
Brand: MM
Price: 463 USD
Availability: InStock

for use in chromatin immunoprecipitations (ChIP assays)

Synonym(s):

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

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Size/SKU	Availability	Price
2.5 mL	Check Cart for Availability	$463.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

eCl@ss:

32160405

Form:

liquid

Biological source:

Staphylococcus aureus

$463.00

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Properties

biological source

Staphylococcus aureus

Quality Segment

100

form

liquid

manufacturer/tradename

Upstate^®

technique(s)

ChIP: suitable

suitability

suitable for immunoprecipitation

shipped in

wet ice

Description

General description

Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.

Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Application

Suitable for use in chromatin immunoprecipitation (ChIPs) assays

Physical form

10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.

Analysis Note

Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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1 of 1

This Item	16201	16125	IP02
Sigma-Aldrich 16157 Protein A Agarose/Salmon Sperm DNA, 2.5 mL	Sigma-Aldrich 16201 Protein G Agarose/Salmon Sperm DNA, 2.5 mL	Millipore 16125 Protein A Agarose	Millipore IP02 Protein A Agarose Suspension
description for use in chromatin immunoprecipitations (ChIP assays)	description for use in chromatin immunoprecipitations (ChIP assays)	description Protein A Agarose Beads for the purification of human, mouse & rabbit immunoglobins.	description Protein A Agarose Suspension designed for immunoprecipitation applications
biological source Staphylococcus aureus	biological source -	biological source -	biological source -
technique(s) ChIP: suitable	technique(s) ChIP: suitable	technique(s) immunoprecipitation (IP): suitable, western blot: suitable	technique(s) -
form liquid	form liquid	form liquid	form slurry (Liquid)
suitability suitable for immunoprecipitation	suitability suitable for immunoprecipitation	suitability -	suitability -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Quality Level 100	Quality Level 100	Quality Level -	Quality Level -

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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