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Anti-Histidine Tagged Antibody, clone 4D11, biotin conjugate

clone 4D11, Upstate®, from mouse

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eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

conjugate

biotin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

4D11, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG2aκ

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HRG(3273)

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05-53116-25416-170
conjugate

biotin conjugate

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ALEXA FLUOR 488

conjugate

biotin conjugate

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

4D11, monoclonal

clone

4D11, monoclonal

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4D11, monoclonal

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manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

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Specificity

Recombinant proteins containing either an amino or carboxyl-terminal HHHHHHGS epitope tag, which is encoded by most His6 tag expression vectors. NOTE: The [GS] amino acids on the epitope tag are required for antibody recognition.

Immunogen

N-terminal His-tagged and truncated TRAP protein (amino acids 26-248) from P. falciparum expressed in E. coli. (catalog #05-531), clone 4D11.

Application

Anti-Histidine Tagged Antibody (anti His antibody), clone 4D11, biotin conjugate is an antibody against Histidine Tagged for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Routinely evaluated in western blot to detect recombinant N-terminal and C-terminal His-tagged proteins

Target description

The apparent molecular weight is related to the tagged protein.

Physical form

PBS, 0.05% sodium azide before the addition of glycerol to 30%
Protein G Purified

Storage and Stability

1 year at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal.
Andreas Stein,Andre Kiesewetter
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences null
Jie He et al.
Oncology reports, 26(5), 1305-1313 (2011-07-20)
In the present study, we investigated the prognostic roles of the O6-methylguanine-DNA methyltransferase (MGMT) gene methylation status, the protein profiles of MGMT, and the glioma stem cell (GSC) marker CD133 in malignant glioma resistance to radiotherapy. The proliferation of glioma
Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro.
Guohui Zhao,Jun Liu,Xiang Liu,Min Chen,Houcheng Zhang,Peng George Wang
Biochemical and biophysical research communications null
K J Robson et al.
The EMBO journal, 14(16), 3883-3894 (1995-08-15)
Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much
Maya Remington et al.
Neuro-oncology, 11(1), 22-32 (2008-09-25)
First-line therapy for patients with glioblastoma multiforme includes treatment with radiation and temozolomide (TMZ), an oral DNA alkylating chemotherapy. Sensitivity of glioma cells to TMZ is dependent on the level of cellular O(6)-methylguanine-DNA methyltransferase (MGMT) repair activity. Several common coding-region

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