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MilliporeSigma

16-232

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate

clone DM1A, Upstate®, from mouse

Synonym(s):

Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, tubulin, alpha 1a, tubulin, alpha 3, tubulin, alpha, brain-specific

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100 μG
$653.00

$653.00

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

DM1A, monoclonal

species reactivity

rat, mouse, guinea pig, human, pig, bovine, avian

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_006000

UniProt accession no.

Q71U36

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

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This Item
05-82905-829-AF64705-829-AF555
Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate clone DM1A, Upstate®, from mouse

Sigma-Aldrich

16-232

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate

Anti-α-Tubulin Antibody, clone DM1A clone DM1A, Upstate®, from mouse

Sigma-Aldrich

05-829

Anti-α-Tubulin Antibody, clone DM1A

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 647 conjugate clone DM1A, from mouse, ALEXA FLUOR™ 647

Sigma-Aldrich

05-829-AF647

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 647 conjugate

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 555 Conjugate clone DM1A, from mouse, ALEXA FLUOR™ 555

Sigma-Aldrich

05-829-AF555

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 555 Conjugate

conjugate

ALEXA FLUOR™ 488

conjugate

-

conjugate

ALEXA FLUOR™ 647

conjugate

ALEXA FLUOR™ 555

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

Gene Information

-

species reactivity

rat, mouse, guinea pig, human, pig, bovine, avian

species reactivity

avian, pig, rat, bovine, mouse, human, guinea pig

species reactivity

guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian

species reactivity

guinea pig, gerbil, mouse, bovine, avian, human, rat, porcine

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

DM1A, monoclonal

clone

DM1A, monoclonal

clone

DM1A, monoclonal

clone

DM1A, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

General description

50-60 kDa
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Immunogen

Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Application

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
Western Blot Analysis:
2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.

Biochem/physiol Actions

Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.

Physical form

Alexa Fluor™ 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide.
Liquid at 2-8°C.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Do Not Freeze. Do not store the material diluted.
For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor™ 488-conjugate.
Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Simon Schneider et al.
eLife, 12 (2023-11-28)
Cylicins are testis-specific proteins, which are exclusively expressed during spermiogenesis. In mice and humans, two Cylicins, the gonosomal X-linked Cylicin 1 (Cylc1/CYLC1) and the autosomal Cylicin 2 (Cylc2/CYLC2) genes, have been identified. Cylicins are cytoskeletal proteins with an overall positive
Yin-Wei Kuo et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(12), 5533-5541 (2019-03-07)
The remodeling of the microtubule cytoskeleton underlies dynamic cellular processes, such as mitosis, ciliogenesis, and neuronal morphogenesis. An important class of microtubule remodelers comprises the severases-spastin, katanin, and fidgetin-which cut microtubules into shorter fragments. While severing activity might be expected
Dhanusha A Nalawansha et al.
Journal of the American Chemical Society, 144(12), 5594-5605 (2022-03-22)
Targeted protein degradation (TPD) by PROTACs is a promising strategy to control disease-causing protein levels within the cell. While TPD is emerging as an innovative drug discovery paradigm, there are currently only a limited number of E3 ligase:ligand pairs that
Naila Umer et al.
Frontiers in cell and developmental biology, 9, 749559-749559 (2021-12-07)
Profilins (PFNs) are key regulatory proteins for the actin polymerization in cells and are encoded in mouse and humans by four Pfn genes. PFNs are involved in cell mobility, cell growth, neurogenesis, and metastasis of tumor cells. The testes-specific PFN3
cAMP, tubulin, axonal transport, and regeneration.
Liu, Howard H and Brady, Scott T
Experimental neurology, 189, 199-203 (2004)
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Questions

  1. What is the recommended dilution for flow cytometry, the product page states "0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6 . . . cells". Is this a 1:500 dilution (eg 100 ug / 0.2ug per test)?

    1 answer

    1. The datasheet from a recent lot, which includes the information you requested, indicates that the antibody is supplied at a concentration of 0.5 mg/mL in a total volume of 200 µL, providing 100 µg of antibody per vial.

      Based on our recommended protocol of using 0.2 µg of antibody per ~2.5 × 10⁵ cells, each vial is expected to provide approximately 500 tests. However, please note that optimization may be required depending on the specific cell type and any variations in cell number or assay volume used in your workflow.

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