Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
This antibody recognizes tyrosine-phosphorylated proteins from all species
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.
Research Sub Category
General Post-translation Modification
100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Protein G-Sepharose Chromatography
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
4G10 is a registered trademark of Upstate Group, Inc.
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