16-316

Anti-Phosphotyrosine Antibody, 4G10^® Platinum, HRP Conjugate

Upstate^®, from mouse, peroxidase conjugate

• Synonym(s): phosphotyrosine
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: peroxidase conjugate
• antibody product type: primary antibodies
• clone: 4G10®, monoclonal; PY20, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG2b
• shipped in: wet ice
• Gene Information: human ... PID1(55022)

Description

General description

The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone.

Application

Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.

Quality

Routinely evaluated by western blot.

Target description

Varies

Physical form

100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10 (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% Kathon and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany