16-455

Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate

clone 4A4, Upstate^®, from mouse

• Synonym(s): 4A4, anti-phosphoserine, phosphoserine antibody, pan-phosphoserine
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: peroxidase conjugate
• Clone: 4A4, monoclonal
• Application: WB
• Citations: 2

Properties

• biological source: mouse
• conjugate: peroxidase conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4A4, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer)
• Quality Level: 100

Description

General description

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotrans-mission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that over 95% of all phosphorylation events occur on serine and threonine residues.

Varies

Immunogen

Epitope: phosphoserine

Phosphoserine conjugated to KLH.

Application

Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate is an antibody against Phosphoserine for use in WB.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Biochem/physiol Actions

Recognizes proteins containing phosphoserine residues, regardless of origin.

Physical form

Protein G Purified

100 μL of protein G purified mouse IgG1 in PBS containing preservative and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:4000 in 10 mL blocking buffer. Liquid.

Preparation Note

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids