Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays.
10 , 50 reactions
This blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays.
Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.
Used to detect/quantify: Protein A+G
Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP® A Kit (Cat. #17-610).
Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze.
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
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