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171536

Sigma-Aldrich

AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, S. frugiperda

AMPK (α1/β1/γ1), His•Tag, Human, Recombinant, is a full length enzyme. Consists of subunits A1, B1, & G1, each fused at the C-terminus to a His•Tag sequence & co-expressed in S. frugiperda.

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recombinant

expressed in insect cells (S. frugiperda)

Quality Level

assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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This Item
171539325896382184
recombinant

expressed in insect cells (S. frugiperda)

recombinant

-

recombinant

-

recombinant

expressed in insect cells (S. frugiperda)

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1).
M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)

Warning

Toxicity: Standard Handling (A)

Physical form

In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Scott J.W., 2007. EMBO J26, 806.
Minokoshi Y et al. 2004. Nature428, 569.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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