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171539

Sigma-Aldrich

AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda

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Synonym(s):
AMP Kinase (α2/β1/γ1)

assay

≥75% (SDS-PAGE)

Quality Level

form

liquid

specific activity

≥270 nmol/min-mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

Kinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.

Physical form

In 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Other Notes

Foretz, M., et al. 2005. Diabetes54, 1331.
Viollet, B., et al. 2003. Biochem. Soc. Trans.31, 216.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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