17-286

Cdc42 Activation Assay Kit

Non-radioactive cdc42 Activation Assay Kit can be used to precipitate cdc42-GTP from cell lysates subjected to various treatments & detection by a cdc42 specific monoclonal antibody.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 100
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable; affinity binding assay: suitable
• NCBI accession no.: NM_001039802.1; NM_001791.3; NM_044472.2
• UniProt accession no.: P60953
• detection method: chemiluminescent
• shipped in: wet ice
• Gene Information: human ... CDC42(998)

Description

Packaging

Kit capacity: 30 assays

Components

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Anti-cdc42

Quality

Routinely evaluated by immunoprecipitating cdc42-GTP from 3T3/NIH cell lysate that has been loaded in vitro with GTPγS. Subsequent detection in western immunoblot using anti-cdc42.

Linkage

Replaces: SGT430

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H318,H410
• pcodes: P273 - P280 - P305 + P351 + P338 - P391 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
• Storage Class: 10 - Combustible liquids