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MilliporeSigma

17-295

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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1 KIT

$607.00

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.41
eCl@ss:
32161000

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manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Quality Level

100

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This Item
17-40917-40817-245
Chromatin Immunoprecipitation (ChIP) Assay Kit Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Sigma-Aldrich

17-295

Chromatin Immunoprecipitation (ChIP) Assay Kit

EZ-Magna ChIP® G - Chromatin Immunoprecipitation Kit Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic G beads. Control primers included.

Sigma-Aldrich

17-409

EZ-Magna ChIP® G - Chromatin Immunoprecipitation Kit

EZ-Magna ChIP® A - Chromatin Immunoprecipitation Kit Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads. Control primers included.

Sigma-Aldrich

17-408

EZ-Magna ChIP® A - Chromatin Immunoprecipitation Kit

Sigma-Aldrich

Sigma-Aldrich

17-245

Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

manufacturer/tradename

EZ-ChIP

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Upstate®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Analysis Note

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Other Notes

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

target_organs

Respiratory Tract

wgk

WGK 3

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - ED ENV 1 - Eye Irrit. 2 - STOT RE 2 Inhalation

Certificates of Analysis (COA)

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Adjacent sequence controls the response polarity of nitric oxide-sensitive Sp factor binding sites
Zhang, J., et al
The Journal of Biological Chemistry, 278, 29192-29200 (2003)
D Pannell et al.
The EMBO journal, 19(21), 5884-5894 (2000-11-04)
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence
A novel cis-acting element in Her2 promoter regulated by Stat3 in mammary cancer cells
Qian, Lu, et al
Biochemical and biophysical research communications null
M Vignali et al.
The EMBO journal, 19(11), 2629-2640 (2000-06-03)
We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro competition assay was developed to acetylate and transcribe a nucleosomal array template in the presence
Seed-based systematic discovery of specific transcription factor target genes.
Mrowka, R; Bluthgen, N; Fahling, M
FEBS Journal null
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