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Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

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Synonym(s):
Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation
eCl@ss:
32161000
NACRES:
NA.41

Quality Level

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

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manufacturer/tradename

EZ-ChIP

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Magna ChIP®

manufacturer/tradename

Upstate®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Components

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Quality

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Adjacent sequence controls the response polarity of nitric oxide-sensitive Sp factor binding sites
Zhang, J., et al
The Journal of Biological Chemistry, 278, 29192-29200 (2003)
Demethylase activity is directed by histone acetylation
Cervoni, N. and Szyf, M.
The Journal of Biological Chemistry, 276, 40778-40787 (2001)
Epigenetic regulation of the endothelial nitric oxide synthase gene in persistent pulmonary hypertension of the newborn rat.
Xu, XF; Ma, XL; Shen, Z; Wu, XL; Cheng, F; Du, LZ
Journal of Hypertension null
Orexin signaling mediates the antidepressant-like effect of calorie restriction.
Lutter, M; Krishnan, V; Russo, SJ; Jung, S; McClung, CA; Nestler, EJ
The Journal of Neuroscience null
Increased expression of hepatocyte nuclear factor 6 stimulates hepatocyte proliferation during mouse liver regeneration.
Yongjun Tan, Yuichi Yoshida, Douglas E Hughes, Robert H Costa
Gastroenterology null

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