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17-344

H2A.X Phosphorylation Assay Kit (Flow Cytometry)

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.

Synonym(s):

Assay for H2A.X, Flow Cytometry Assay Kit, H2A.X Phosphorylation Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32
eCl@ss:
32161000

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Product Name

H2A.X Phosphorylation Assay Kit (Flow Cytometry), The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable
flow cytometry: suitable

NCBI accession no.

NM_002105.2

UniProt accession no.

P16104

detection method

fluorometric

shipped in

dry ice

Quality Level

100

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This Item
17-327APT11005-636-AF488
H2A.X Phosphorylation Assay Kit (Flow Cytometry) The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.

Sigma-Aldrich

17-344

H2A.X Phosphorylation Assay Kit (Flow Cytometry)

H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)

Sigma-Aldrich

17-327

H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)

Apo-Direct TUNEL Assay Kit The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

Sigma-Aldrich

APT110

Apo-Direct TUNEL Assay Kit

Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor™ 488 Conjugate Antibody clone JBW301, from mouse, ALEXA FLUOR™ 488

Sigma-Aldrich

05-636-AF488

Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor™ 488 Conjugate Antibody

technique(s)

activity assay: suitable, flow cytometry: suitable

technique(s)

ELISA: suitable

technique(s)

flow cytometry: suitable

technique(s)

immunocytochemistry: suitable

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

ApoDIRECT, Chemicon®

manufacturer/tradename

-

NCBI accession no.

NM_002105.2

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

NP_002096

UniProt accession no.

P16104

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P16104

detection method

fluorometric

detection method

chemiluminescent

detection method

fluorometric

detection method

-

shipped in

dry ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

Analysis Note

phosphorylation of Histone H2A.X

Application

Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X.

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.

Other Notes

Anti-phospho H2A.X, FITC conjugate

Normal Mouse IgG, FITC conjugate (Cat.# 12-487)

16X Fixation Solution

10X Permeabilization Solution

10X Wash Solution

Packaging

100 assays

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Runtao Zhong et al.
RSC advances, 10(49), 29311-29319 (2020-08-07)
Immunofluorescence (IF) is a common method used in cell biology. The conventional protocol for IF staining is time and labor-intensive, operator dependent and reagent-consuming. Magnetic Bead (MB)-based microdevices are frequently utilized in cellular assays, but integration of simple and efficient
Yu Wang et al.
Mutation research, 734(1-2), 20-29 (2012-05-09)
Berberine has been shown to possess anti-tumor activity against a wide spectrum of cancer cells. It inhibits cancer cell proliferation by inducing cell cycle arrest, at G1 and/or G2/M, and apoptosis. While it has been documented that berberine induces G1
Angelo Fortunato et al.
PLoS biology, 19(11), e3001471-e3001471 (2021-11-18)
Trichoplax adhaerens is the simplest multicellular animal with tissue differentiation and somatic cell turnover. Like all other multicellular organisms, it should be vulnerable to cancer, yet there have been no reports of cancer in T. adhaerens or any other placozoan.
Amy D Guertin et al.
Molecular cancer therapeutics, 12(8), 1442-1452 (2013-05-24)
Inhibition of the DNA damage checkpoint kinase WEE1 potentiates genotoxic chemotherapies by abrogating cell-cycle arrest and proper DNA repair. However, WEE1 is also essential for unperturbed cell division in the absence of extrinsic insult. Here, we investigate the anticancer potential
Amy D Guertin et al.
Cancer cell international, 12(1), 45-45 (2012-11-15)
Inhibition of kinases involved in the DNA damage response sensitizes cells to genotoxic agents by abrogating checkpoint-induced cell cycle arrest. CHK1 and WEE1 act in a pathway upstream of CDK1 to inhibit cell cycle progression in response to damaged DNA.
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