17-701

EZ-Magna RIP^® RNA-Binding Protein Immunoprecipitation Kit

12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads

Properties

• Quality Level: 100
• packaging: kit of 12 assay(s)
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable (RNA-binding protein immunoprecipitation)

Description

General description

RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

Application

RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads. Control primers included.

Features and Benefits

• Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
• RNAse inhibitors and RNAse-free reagents
• Positive and negative controls

Packaging

Three boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Includes positive control antibody and control primers.

Components

• Magnetic Beads Protein A/G
• RIP Wash Buffer
• RIP Lysis Buffer
• 0.5 M EDTA
• 10% SDS
• Salt Solution I
• Salt Solution II
• Precipitate Enhancer
• Normal Mouse IgG
• Rabbit IgG Purified
• Protease Inhibitor Cocktail 200X
• RNase Inhibitor
• Proteinase K (10 mg/mL)
• Nuclease free water
• Positive Control Antibody (Anti-SNRNP70)
• Control Primers

Storage and Stability

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.

Legal Information

MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302 - H315 - H319 - H412
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3