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Sigma-Aldrich

Apoptosis Inhibitor

The Apoptosis Inhibitor, also referenced under CAS 54135-60-3, controls the biological activity of Apoptosis. This small molecule/inhibitor is primarily used for Cancer applications.

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Synonym(s):
Apoptosis Inhibitor, M50054, 2,2ʹ-Methylenebis(1,3-cyclohexanedione)
Empirical Formula (Hill Notation):
C13H16O4
CAS Number:
Molecular Weight:
236.26

Quality Level

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

off-white

solubility

DMSO: 100 mg/mL
ethanol: 20 mg/mL

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C13H16O4/c14-10-3-1-4-11(15)8(10)7-9-12(16)5-2-6-13(9)17/h8-9H,1-7H2

InChI key

ILUMEPMGPCKGHH-UHFFFAOYSA-N

General description

A cell-permeable inhibitor of apoptosis induction (IC50 = 67 µg/ml in FasL-stimulated WC8 cells, 130 µg/ml in etoposide-stimulated U937 cells). Effects attributable to the inhibition of caspase-3 activation but does not directly inhibit caspase-3 even at 1 mg/ml.
A cell-permeable, bis(cyclohexane-1,3-diketone) that is shown to inhibit apoptosis induction in FasL-stimulated WC8 cells (IC50 = 67 µg/ml) and etoposide-stimulated U937 cells (IC50 = 130 µg/ml). The anti-apoptotic effects have been shown to be attributable to inhibition of caspase-3 activation (IC50 = 334 nM), however it does not exhibit any direct inhibition of caspase-3 enzymatic activity. Shown to inhibit the elevation of the liver enzymes, alanine aminotransferase and aspartate aminotransferase, in a murine model of anti-Fas-induced hepatitis. Topical application has been shown to reduce chemotherapy-induced alopecia (hair loss) in newborn rats.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
apoptosis induction
Product does not compete with ATP.
Reversible: no
Target IC50: 67 µg/ml in FasL-stimulated WC8 cells, 130 µg/ml in etoposide-stimulated U937 cells

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Tsuda, T., et al. 2001. Eur. J. Pharmacol.433, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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