A working dilution of 1:20,000 to 1:100,000 is suggested for ELISA.
Please see the link below to review the available Certificates of Analysis:
https://www.emdmillipore.com/US/en/product/Protein-A-HRP-conjugate,MM_NF-18-160#anchor_COA
18-160
Protein A, HRP conjugate
1 mg Protein A, HRP conjugate for Dot Blot, ELISA, Western Blotting & IHC.
Synonym(s):
detection reagent
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About This Item
UNSPSC Code:
41105332
eCl@ss:
32160405
NACRES:
NB.22
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$215.00
Check Cart for Availability
form
solid
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable
shipped in
wet ice
1 of 4
This Item | 18-152 | WBULP-100ML | WBULP-20ML |
|---|---|---|---|
| technique(s) ELISA: suitable, immunohistochemistry: suitable, dot blot: suitable, western blot: suitable | technique(s) ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) dot blot: suitable, immunoblotting: suitable, western blot (chemiluminescent): suitable | technique(s) dot blot: suitable, immunoblotting: suitable, western blot (chemiluminescent): suitable |
| form solid | form liquid | form - | form - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename - | manufacturer/tradename - |
Application
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.
Physical form
chromatographically pure Protein A conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10 mg/ml BSA- Immunoglobulin and Protease free
Storage and Stability
Lyophilized: Stable for 2 years at -20°C from date of shipment. Rehydrated: Stable for 6 months at -20°C.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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What concentration of use do you suggest for ELISA?
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How to rehydrate? Please give me some suggestions about Protein A (Product No.18-160). Thank you!
1 answer-
To rehydrate, add 500 µL sterile, distilled water to make a 2 mg/mL stock solution. Solutions are stable for 6 months at -20°C. Aliquot to avoid repeated freeze/thaw cycles.
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