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MilliporeSigma

18-160

Millipore

Protein A, HRP conjugate

1 mg Protein A, HRP conjugate for Dot Blot, ELISA, Western Blotting & IHC.

Synonym(s):

detection reagent

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1 MG
$215.00

About This Item

UNSPSC Code:
41105332
eCl@ss:
32160405
NACRES:
NB.22

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$215.00


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Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

solid

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable

shipped in

wet ice

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This Item
18-152WBULP-100MLWBULP-20ML
technique(s)

ELISA: suitable, immunohistochemistry: suitable, dot blot: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

dot blot: suitable, immunoblotting: suitable, western blot (chemiluminescent): suitable

technique(s)

dot blot: suitable, immunoblotting: suitable, western blot (chemiluminescent): suitable

form

solid

form

liquid

form

-

form

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

Application

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.

Physical form

chromatographically pure Protein A conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10 mg/ml BSA- Immunoglobulin and Protease free

Storage and Stability

Lyophilized: Stable for 2 years at -20°C from date of shipment. Rehydrated: Stable for 6 months at -20°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  
  1. What concentration of use do you suggest for ELISA?

    1 answer
    1. A working dilution of 1:20,000 to 1:100,000 is suggested for ELISA.
      Please see the link below to review the available Certificates of Analysis:
      https://www.emdmillipore.com/US/en/product/Protein-A-HRP-conjugate,MM_NF-18-160#anchor_COA

      Helpful?

  2. How to rehydrate? Please give me some suggestions about Protein A (Product No.18-160). Thank you!

    1 answer
    1. To rehydrate, add 500 µL sterile, distilled water to make a 2 mg/mL stock solution. Solutions are stable for 6 months at -20°C. Aliquot to avoid repeated freeze/thaw cycles.

      Helpful?

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