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  • 189730
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189730

Sigma-Aldrich

Streptavidin

Synonym(s):
Streptavidin, Avidin, Streptomyces avidinii
CAS Number:
MDL number:

form

lyophilized solid

Quality Level

specific activity

≥10 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

shipped in

ambient

storage temp.

−20°C

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370951623003PHR2687
Sigma-Aldrich

Sigma-Aldrich

189730

Streptavidin

Staurosporine VETRANAL®, analytical standard

Supelco

37095

Staurosporine

Streptomycin sulfate United States Pharmacopeia (USP) Reference Standard

USP

1623003

Streptomycin sulfate

Streptomycin Sulfate certified reference material, pharmaceutical secondary standard

Supelco

PHR2687

Streptomycin Sulfate

form

lyophilized solid

form

-

form

-

form

solid

specific activity

≥10 units/mg protein

specific activity

-

specific activity

-

specific activity

-

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

-

storage condition

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-10 to -25°C

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

-

General description

A high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
A high-affinity biotin binding protein.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.

Other Notes

Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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