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189730

Sigma-Aldrich

Streptavidin

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Synonym(s):
Streptavidin, Avidin, Streptomyces avidinii
CAS Number:
MDL number:

form

lyophilized solid

Quality Level

specific activity

≥10 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

shipped in

ambient

storage temp.

−20°C

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This Item
S0677A7294A7419
vibrant-m

189730

Streptavidin

vibrant-m

A7294

Avidin–Alkaline Phosphatase

vibrant-m

A7419

Avidin–Peroxidase

specific activity

≥10 units/mg protein

specific activity

-

specific activity

-

specific activity

-

shipped in

ambient

shipped in

-

shipped in

wet ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

-

storage condition

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

A high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
A high-affinity biotin binding protein.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.

Other Notes

Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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