MilliporeSigma
All Photos(1)

Documents

19-144

Sigma-Aldrich

Phorbol 12-Myristate 13-Acetate

All Photos(1)
Synonym(s):
12-O-Tetradecanoylphorbol 13-acetate, 4β,9α,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate, PMA, TPA
eCl@ss:
32160405
NACRES:
NA.41

form

solid

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

shipped in

wet ice

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
1614385CDS002964CDS021337
Sigma-Aldrich

Sigma-Aldrich

19-144

Phorbol 12-Myristate 13-Acetate

USP

USP

1614385

Sodium Myristoyl Sarcosinate

Methyl (4-aminophenyl)acetate AldrichCPR

Sigma-Aldrich

CDS002964

Methyl (4-aminophenyl)acetate

(2S)-2-Methylpyrrolidine-2-methanol

Sigma-Aldrich

CDS021337

(2S)-2-Methylpyrrolidine-2-methanol

manufacturer/tradename

Upstate®

manufacturer/tradename

USP

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

activity assay: suitable (kinase)

technique(s)

-

technique(s)

-

technique(s)

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

Application

Phorbol 12-Myristate 13-Acetate is suitable as an inducer of cell differentiation in monocytic cell lines such as U937 and THP-1.

Biochem/physiol Actions

Phorbol 12-Myristate 13-Acetate is a tumor-promoting phorbol ester. It regulates various processes such as gene transcription, cellular growth and differentiation, apoptosis, the immune response, and receptor desensitization via activating protein kinase C (PKC) signaling pathways. It promotes differentiation of human monocytic THP-1 cells to functional macrophages.

Physical form

C36H56O8

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

Interferon-γ human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), lyophilized powder, suitable for cell culture

Sigma-Aldrich

I3265

Interferon-γ human

Menadione sodium bisulfite ≥95% (TLC)

Sigma-Aldrich

M5750

Menadione sodium bisulfite

Etoposide synthetic, 95.0-105.0%, powder

Sigma-Aldrich

E1383

Etoposide

Sigma-Aldrich

Sigma-Aldrich

D4693

Dispase® II

Lipopolysaccharides from Escherichia coli O111:B4 purified by phenol extraction

Sigma-Aldrich

L2630

Lipopolysaccharides from Escherichia coli O111:B4

In vitro studies on the mode of action of the phorbol esters, potent tumor promoters: part 1.
Blumberg, P M
Critical Reviews in Toxicology, 8, 153-197 (1980)
Phorbol 12-myristate 13-acetate upregulates cyclooxygenase-2 expression in human pulmonary epithelial cells via Ras, Raf-1, ERK, and NF-kappaB, but not p38 MAPK, pathways.
Chang, et al.
Cellular Signalling, 17, 299-310 (2018)
Kassim Traore et al.
Leukemia research, 29(8), 863-879 (2005-06-28)
Human monocytic THP-1 cells can be induced to differentiate to macrophages when treated with phorbol 12-myristate 13-acetate (PMA). It is understood that before initiating cell differentiation, PMA treatment must first induce an inhibition of cell growth. Since the initial biochemical
J F Valdés López et al.
Morphologie : bulletin de l'Association des anatomistes, 102(338), 205-218 (2018-08-05)
Phorbol-12-myristate-13-acetate (PMA) and 1,25-dihydroxyvitamin D3 (VD3) are stimuli commonly used to induce macrophage differentiation in monocytic cell lines, but the extent of differentiation in comparison to primary tissue macrophages is unclear. Here, we examine the morphological/phenotypic markers associated with differentiation
12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced increase in depressed white blood cell counts in patients treated with cytotoxic cancer chemotherapeutic drugs.
Han, Z T, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 5362-5365 (1998)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service