194434

Sigma-Aldrich

ABTS Chromophore

Synonym(s):
ABTS Chromophore
Empirical Formula (Hill Notation):
C18H18N4O6S4
Molecular Weight:
514.62

storage conditions

+2C to +8C

form

solution

Quality Level

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

clearvery pale yellow

shipped in

ambient

General description

Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.
Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.

Packaging

100 ml in Glass bottle

Warning

Toxicity: Irritant (B)

Physical form

Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.

Reconstitution

ABTS solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.

Other Notes

Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.

Legal Information

ABTS is a trademark of Boehringer Mannheim Corp.
ABTS is a trademark of Roche
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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