Stock solutions of this product can be made in DMSO to a concentration of 100 µM. Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 year at -20°C. Just before use, the stock solution should be serially diluted in DMSO to a working stock that is at a 1000-fold higher concentration than the concentration desired in the final aqueous reaction mix or culture medium.
196000
Bafilomycin A1
from Streptomyces griseus, ≥90% (HPLC), film, V-ATPase inhibitor, Calbiochem
Synonym(s):
Bafilomycin A1, Streptomyces griseus
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10 μG
$222.00
1 SET
$549.00
$222.00
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About This Item
Empirical Formula (Hill Notation):
C35H58O9
CAS Number:
Molecular Weight:
622.83
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
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Product Name
Bafilomycin A1, Streptomyces griseus, Bafilomycin A1, CAS 88899-55-2, acts as a highly potent and specific inhibitor of vacuolar-type H+-ATPase (Ki = 500 pM). Blocks the fusion of autophagosome with lysosome.
Quality Level
assay
≥90% (HPLC)
form
film
shelf life
3 yr
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
solubility
DMSO: 100 μM
ethanol: soluble
storage temp.
−20°C
SMILES string
O1[C@@H]([C@H]([C@@H](C[C@]1(O)[C@H]([C@H](O)[C@@H]([C@H]2OC(=O)\C(=C\C(=C\[C@H]([C@H]([C@H](C\C(=C\C=C\[C@@H]2OC)\C)C)O)C)\C)\OC)C)C)O)C)C(C)C
InChI
1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
InChI key
XDHNQDDQEHDUTM-JQWOJBOSSA-N
1 of 4
This Item | 676377 | 5.08409 | B1793 |
|---|---|---|---|
| assay ≥90% (HPLC) | assay ≥93% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) |
| form film | form crystalline solid | form liquid | form film |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename - |
| storage temp. −20°C | storage temp. 10-30°C | storage temp. −20°C | storage temp. −20°C |
| storage condition OK to freeze, protect from light | storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light | storage condition - |
General description
A macrolide antibiotic that acts as a specific inhibitor of vacuolar-type H+-ATPase (V-type; Ki = 500 pM). A valuable tool for distinguishing among different types of ATPases. Blocks lysosomal cholesterol trafficking in macrophages and is known to interfere with pH regulation in brain cells. Exhibits cytotoxic effects on a number of cell lines in a cell viability assay. Reported to selectively inhibit β-secretase, an enzyme involved in the processing of amyloid precursor protein (APP). The InSolution format with a purity of ≥97% by HPLC in 90% DMSO is also available (Cat. No. 508409).
Note: 1 set = 3 x 10 µg
Biochem/physiol Actions
Primary Target
vacuolar type H+ ATPase
vacuolar type H+ ATPase
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 year at -20°C.
Other Notes
Sinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad. Sci. USA96, 11049.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem.270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun.269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem.269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem.269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res.9, 1575.
Drose, S., et al. 1993. Biochemistry32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem.268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7972.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem.270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun.269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem.269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem.269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res.9, 1575.
Drose, S., et al. 1993. Biochemistry32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem.268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7972.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Carcinogenic / Teratogenic (D)
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
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