203783

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203783

5-Bromo-4-chloro-3-indolyl-β-D-glucuronic Acid, Cyclohexylammonium Salt

Substrate for β-glucuronidase that produces insoluble, intense indigo-blue chromophore (615 nm) after enzymatic hydrolysis.

Synonym(s):

5-Bromo-4-chloro-3-indolyl-β-D-glucuronic Acid, Cyclohexylammonium Salt, X-GlcA, X-Gluc, BCIG, BCI-β-D-glucuronide, BCI-glucuro, X-Glucuro, CHA-X-Gluc

Empirical Formula (Hill Notation):

C₂₀H₂₆BrClN₂O₇

CAS Number:

18656-96-7

Molecular Weight:

521.79

Quality Level

100

Assay

≥98% (TLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
protect from light

color

white

solubility

2% DMF: 25 mg/mL

shipped in

ambient

storage temp.

−20°C

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This Item	VBP00170	370660	407125
Sigma-Aldrich 203783 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic Acid, Cyclohexylammonium Salt	Sigma-Aldrich VBP00170 4-(5-Bromo-1H-indol-3-yl)-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester	Sigma-Aldrich 370660 Guanosine 3′,5′-cyclic Monophosphate, N²,2′-O-Dibutyryl-, Sodium Salt	Sigma-Aldrich 407125 D-myo-Inositol 1,2,3,4,5,6-Hexakisphosphate, Dodecasodium Salt, Zea mays
assay ≥98% (TLC)	assay -	assay ≥95% (HPLC)	assay ≥98% (HPLC)
form solid	form solid	form solid	form solid
manufacturer/tradename Calbiochem^®	manufacturer/tradename -	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®
storage condition OK to freeze, protect from light	storage condition -	storage condition OK to freeze, desiccated (hygroscopic)	storage condition OK to freeze, desiccated (hygroscopic)
color white	color -	color white	color white to off-white
solubility 2% DMF: 25 mg/mL	solubility -	solubility ethanol: soluble, water: soluble	solubility water: 1 mg/mL

General description

Substrate for β-glucuronidase that produces insoluble, intense indigo-blue chromophore (615 nm) after enzymatic hydrolysis. Useful for a variety of applications, including use in bacterial detection systems (e.g. E. coli).

Warning

Toxicity: Standard Handling (A)

Other Notes

Jefferson, R.A., et al. 1986. Proc. Natl. Acad. Sci. USA83, 8447.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificate of Quality