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biological source
(Synthetic chemical)
Quality Level
form
(Clear to pale yellow liquid)
storage condition
OK to freeze
storage temp.
2-8°C
General description
BCIP/NBT provided as a stable single component, ready-to-use substrate, sterile-filtered through a 0.22 µm filter. Specifically designed for immunoblotting techniques.BCIP/NBT is the substrate of choice for alkaline phosphatase-based immunoblotting procedures. Hydrolysis of the phosphate ester of BCIP by alkaline phosphatase yields powerful reducing compounds that react rapidly with NBT and convert it to the insoluble NBT-formazan. This reaction product is readily visible on the blotting membrane as dark purple deposits that do not fade upon drying.
Application
Immunoblotting
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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