204896

Sigma-Aldrich

Complement C8, Human

Complement C8, Human, CAS 80295-58-5, is a native glycoprotein composed of three non-identical subunits of 64, (α), 64 (β), and 22 kDa (γ).

Synonym(s):
Complement C8, Human
CAS Number:
MDL number:

storage conditions

= -70C

Quality Level

assay

≥85% (SDS-PAGE)

form

liquid

specific activity

≥150,000 C8H50 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

impurities

IgG, IgA, IgM, albumin, C3, C4, C7, C9, trace

shipped in

dry ice

General description

Native, human C8 complement component. Glycoprotein composed of three non-identical subunits of M.W. 64 kDa (α), 64 kDa (β), and 22 kDa (γ). Present in normal human serum at 55 µg/ml. On activation of complement via either the classical or alternative pathway, C5 is cleaved into C5a and C5b fragments. The nascent C5b fragment binds to C6, C7, and C8 resulting in formation of the C5b-8 complex on target surfaces. Each membrane-bound, hydrophobic C5b-8 complex combines with three to six C9 molecules to complete the assembly of the lytic C5b-9 complement membrane attack complex (MAC).
Native, human C8 complement component. Glycoprotein composed of three non-identical subunits of M.W. 64,000 (α), 64,000 (β), and 22,000 (γ). Present in normal human serum at 55 µg/ml. On activation of complement via either the classical or alternative pathway, C5 is cleaved into C5a and C5b fragments. The nascent C5b fragment binds to C6, C7, and C8, resulting in formation of C5b-8 complex on target surfaces. Each membrane-bound, hydrophobic C5b-8 complex combines with three to six C9 molecules to complete the assembly of the lytic C5b-9 complement membrane attack complex (MAC).

Packaging

250 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.2.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Steckel, E.W., et al. 1980. J. Biol. Chem. 255, 11997.
Petersen, B.H., et al. 1976. J. Clin. Invest. 57, 283.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis
Certificate of Origin

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