207005

Calcineurin Assay Kit, Colorimetric

• Synonym(s): Colorimetric, PP2B Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 96 tests
• Quality Level: 100
• manufacturer/tradename: Calbiochem^®
• packaging: pkg of 96-well plate(s)
• storage condition: OK to freeze
• detection method: colorimetric
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

A colorimetric assay kit for measuring calcineurin (PP2B) activity and for inhibitor screening. The assay is performed using RII Phosphopeptide Substrate (Cat. No. 207008).

Features and Benefits

Assay time: 3.5 h

Packaging

1 kit in Glass bottle

Preparation Note

Store all components except the 96-Well Plate (room temperature) at -70°C for the highest stability. In particular, calcineurin must be handled with utmost care in order to retain its maximal enzymatic activity. Thaw it quickly in a water bath, maintained at room temperature, or by rubbing between fingers, then immediately store on an ice bath. Any remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, we recommend to aliquot the calcineurin into separate tubes and then store at -70°C.

Other Notes

Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.

Mondragon, A. et al. 1997. Biochemistry 36, 4934.
Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials