218765

Sigma-Aldrich

Caspase-9 Substrate I, Fluorogenic - Calbiochem

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

Synonym(s):
Caspase-9 Substrate I, Fluorogenic - Calbiochem, Ac-LEHD-AFC
Empirical Formula (Hill Notation):
C33H38F3N7O11
Molecular Weight:
765.69

storage conditions

-20C

Quality Level

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white

solubility

DMSO: 5 mg/mL

fluorescence

λex ~400  nm
λem ~505 nm

shipped in

ambient

storage temp.

−20°C

General description

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Packaging

1 mg in Glass bottle
5 mg in Plastic ampoule

Biochem/physiol Actions

Product does not compete with ATP.
Primary Target
A fluorogenic substrate for caspase-9
Reversible: no
Cell permeable: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Leu-Glu-His-Asp-AFC

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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