218829

Sigma-Aldrich

Caspase-3 Substrate IX, Fluorogenic - Calbiochem

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate.

Synonym(s):
Caspase-3 Substrate IX, Fluorogenic - Calbiochem, (Z-DEVD)₂-Rh 110
Empirical Formula (Hill Notation):
C72H78N10O27
Molecular Weight:
1515.44
Pricing and availability is not currently available.

storage conditions

-20C

Quality Level

assay

≥95% (HPLC)

form

lyophilized solid

mfr. no.

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

DMSO: 1 mg/mL

fluorescence

λex ~485  nm
λem ~535 nm

shipped in

wet ice

General description

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.
A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Useful for high throughput screening applications.

Packaging

1 mg in Plastic ampoule
Packaged under inert gas

Biochem/physiol Actions

Primary Target
A highly sensitive, photostable caspase-3 fluorogenic substrate
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Warning

Toxicity: Standard Handling (A)

Sequence

(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hug, H., et al. 1999. Biochemistry38, 13906.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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