219350
Anti-β-Catenin, Dephospho Mouse mAb (8E4)
lyophilized, clone 8E4, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
8E4, monoclonal
form
lyophilized
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
General description
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.
Immunogen
Mouse
a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
Application
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
Warning
Toxicity: Standard Handling (A)
Physical form
100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
Reconstitution
Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
LiCl-treated SW480 cells (included)
LiCl-treated SW480 cells (included)
Other Notes
SW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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