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Sigma-Aldrich

Coagulation Factor V, Human Plasma

Coagulation Factor V, Human Plasma, CAS 9001-24-5, is a native coagulation factor V that is cleaved by thrombin to yield activated Factor Va that is 50-fold greater activity than its precursor form.

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Synonym(s):
Coagulation Factor V, Human Plasma, Proaccelerin, Labile Factor, Ac-globulin, Ac-g
CAS Number:

Quality Level

Assay

≥95% (SDS-PAGE)

form

liquid

specific activity

≥20 units/mg protein (clotting assay)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

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This Item
F0681F0931233279
Sigma-Aldrich

Sigma-Aldrich

F0681

Factor V human

Sigma-Aldrich

Sigma-Aldrich

F0931

Factor Va human

form

liquid

form

aqueous glycerol solution

form

solution

form

liquid

specific activity

≥20 units/mg protein (clotting assay)

specific activity

≥25 units/mg protein

specific activity

≥1500 units/mg protein

specific activity

≥150 units/mg protein (clotting assay)

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

General description

Native coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca2+, and membrane phospholipids are cofactors that form a "prothrombinase" complex with Factor X, enabling its proteolytic activation of prothrombin to thrombin.
Native coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca2+, and membrane phospholipids are cofactors which form a "prothrombinase" complex with Factor Xa, enabling its proteolytic activation of prothrombin to thrombin.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50% glycerol/H₂O (v/v).

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Do not store below -20°C.

Analysis Note

Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma

Other Notes

Walker, F.J. 1992. J. Biol. Chem.267, 19896.
Monkovic, D.D., and Tracy, P.B. 1990. J. Biol. Chem.265, 17132.
Krishnaswamy, S., et al. 1989. J. Biol. Chem.264, 3160.
Lee, C.D., and Mann, K.G. 1989. Blood73, 185.
Krieg, U.C., et al. 1987. Biochemistry26, 103.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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