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219461

Sigma-Aldrich

Cathepsin K, His•Tag®, Human, Recombinant, E. coli

Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.

CAS Number:
Enzyme Commission number:

Quality Level

assay

≥95% (SDS-PAGE)

form

liquid

specific activity

≥900 mU/mg protein

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His•Tag.
Note: 1 mU = 1 milliunit.
Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag sequence.

Packaging

25 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.

Physical form

In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Delaisse, J.M., et al. 2003. Microsc. Res. Tech.61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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