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228180

Sigma-Aldrich

Cholesterol Esterase, Microbial

Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Synonym(s):
CE, Sterolester Acylhydrolase, Cholesterol Esterase, Microbial
CAS Number:
Enzyme Commission number:
MDL number:

Quality Level

form

powder

specific activity

≥100 units/mg protein
≥70 units/mg dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

light brown

solubility

10 mM potassium phosphate/sodium hydroxide, 0.1% BSA, pH 7.5: soluble

foreign activity

Catalase ≤1.5%

shipped in

ambient

storage temp.

−20°C

General description

Native, microbial cholesterol esterase. Catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Packaging

1000 u in Plastic ampoule
Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol cholesterol ester per min at 37°C, pH 6.5.

Reconstitution

Reconstitute just prior to use. Enzyme will lose activity in solution upon storage.

Other Notes

Allain, C.C., et al. 1974. Clin. Chem.20, 470.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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