The product is supplied as a solution with varying concentration from lot to lot. The lot-specific concentration is reported in the product Certificate of Analysis. A brief review of recent lots indicates a rough concentration of 1 mg/mL, but this may deviate significantly. The reviewed lots range in concentration from 0.948 – 1.32 mg/mL. Please see the link below to request a lot-specific Certificate:
https://www.sigmaaldrich.com/US/en/product/mm/23034m#product-documentation
23-034-M
p53 Protein, 10 µg
Full length, recombinant human p53. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Synonym(s):
Cellular tumour antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumour suppressor p53, TP53
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Check Cart for Availability | $782.00 |
250 μg | Check Cart for Availability | $4,360.00 |
About This Item
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Form:
liquid
Biological source:
human
Recombinant:
expressed in E. coli
biological source
human
Quality Segment
recombinant
expressed in E. coli
form
liquid
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
solubility
water: soluble
GenBank accession no.
storage temp.
−70°C
Gene Information
human ... TP53(120407068)
General description
Application
Ubiquitination Cascade Comoponent: Substrate
Biochem/physiol Actions
p53 is expressed in a wide variety of tissues and plays a major role in preventing tumour development. It is involved in cell cycle regulation, functioning as a trans-activator to negatively regulate cell division by controlling a set of genes required for this process. It acts as a tumour suppressor in many tumour types by stimulating either apoptosis or growth arrest, depending on the physiological circumstances and the cell type. Defects in p53 expression have been implicated in several diseases, including choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, oesophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortical carcinoma.
Physical form
Purified using immobilized metal affinity chromatography.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hi, for product 23-034-M p53 Protein, it is 10 µg of p53 in how many volume of liquid?
1 answer-
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What do “6×His-tagged” and “N-terminal GST-tagged” mean? Which type of tag is present on this full-length p53 (23-034-M, Full length, recombinant human p53) ? Does the tag affect the spectroscopic analysis?
1 answer-
A 6×His tag is a small epitope tag (0.84 kDa) that can be incorporated at the N-terminus, C-terminus, or internal sites of a protein. This full-length recombinant human p53 protein is described as "N-terminal c-Myc, 6His-tagged," indicating the presence of a 6×His tag at the N-terminus, which consists of six histidine residues utilized for affinity purification and detection. A GST tag is much larger (26 kDa), typically added at the N- or C-terminus, and is used to increase protein expression and solubility. There is no GST tag present on this protein. The 6×His tag is suitable for detection, purification, and immobilization, and is unlikely to interfere with most spectroscopic methods.
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