233156

Cleland′s Reagent, Molecular Biology Grade

A protective agent for SH groups.

• Synonym(s): Cleland’s Reagent, Molecular Biology Grade, 1,4-Dithiothreitol, DTT
• Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• MDL number: MFCD00004877
• UNSPSC Code: 12161504
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 100
• description: Merck USA index - 14, 3376
• assay: ≥99% (titration)
• form: powder; solid
• reaction suitability: reagent type: reductant
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 15 mg/mL
• cation traces: heavy metals: ≤0.001%
• absorption: ≤0.100 at 280 nm at 100 mM (1 cm); ≤0.500 at 260 nm at 100 mM (1 cm)
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• InChI key: VHJLVAABSRFDPM-IMJSIDKUSA-N

Description

General description

A protective agent for SH groups.

Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H315,H318
• pcodes: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable