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MilliporeSigma

234405

Complement C5-Depleted Serum, Human

Synonym(s):

Complement Serum

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1 ML

$335.00

$335.00

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25
Form:
liquid
Shipped in:
dry ice

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form

liquid

Quality Level

100

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

impurities

C5 antigen, none detected (Ouchterlony)

shipped in

dry ice

storage temp.

−70°C

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Sigma-Aldrich

234403

Complement C3-Depleted Serum, Human

Complement C1q-Depleted Serum, Human

Sigma-Aldrich

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Complement C1q-Depleted Serum, Human

form

liquid

form

liquid

form

liquid

form

solution

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

impurities

C5 antigen, none detected (Ouchterlony)

impurities

-

impurities

-

impurities

C1q, none detected (Ouchterlony using various dilutions of goat anti-human C1q antiserum.)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

General description

Human serum depleted of complement component C5 by affinity chromatography.
Human serum that has been depleted of complement component C5 by affinity chromatography. Intended for the detection and quantitation of hemolytically active C5 complement component. The classical and alternative pathways are intact except for the specifically depleted component. Recommended assay volume: ≤60 µl

Biochem/physiol Actions

Complement C5-depleted serum from humans produced by affinity chromatography aids the development of the complement cascade up to the desired convertase level. It eliminates the usual time-consuming, expensive, and stepwise formation of enzymatic complexes from purified products. Whole serum usage reduces the risk of protein inactivation during the process. It also promotes better physiological conditions.[1] Complement C5-Depleted Serum is intended for the detection and quantitation of the hemolytically active C5 complement component. Creating complement convertases on erythrocytes enhances a better readout of the assay by spectrophotometric quantification of released hemoglobin.[1]

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 145 mM NaCl, 10 mM sodium phosphate, pH 7.3.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).
Prepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marcin Okroj et al.
PloS one, 7(10), e47245-e47245 (2012-10-17)
C3 and C5 convertases are central stages of the complement cascade since they converge the different initiation pathways, augment complement activation by an amplification loop and lead to a common terminal pathway resulting in the formation of the membrane attack

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