235417

Caspase-3, Human, Recombinant, E. coli

Caspase-3, Human, Recombinant, is expressed as a proenzyme in E. coli and is subsequently cleaved to produce the active enzyme.

• Synonym(s): YAMA, Apopain, CPP32, YAMA, CPP32, Apopain
• UNSPSC Code: 12352202

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: liquid
• specific activity: ≥1450 units/μg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

M.W. 12,000 and 17,000 (heterodimer).

Recombinant, human caspase-3 expressed in E. coli as the proenzyme, which is subsequently cleaved to produce the active enzyme. The active enzyme is a heterodimer with subunit molecular weights of 12 kDa and 19 kDa. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage and inhibitor screening. Caspase-3 is a member of the ICE family of cysteine proteases that is activated during apoptotic signaling events by upstream proteases, including caspase-6, caspase-8 (FLICE) and cytotoxic T-cell-derived granzyme B.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol of the substrate Ac-DEVD-pNA (Cat. No. 235400) per min at 30°C, pH 7.4.

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Izban, K.F., et al. 1999. Am. J. Pathol. 154, 1439.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Mittl, P.R.E., et al. 1997. J. Biol. Chem. 272, 6539.
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2