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239765

Sigma-Aldrich

Cycloheximide

InSolution, 100 mg/mL in DMSO, Suitable for cell culture

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Synonym(s):
InSolution Cycloheximide
Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
Molecular Weight:
281.35
MDL number:

Quality Level

form

solution

manufacturer/tradename

Calbiochem®

concentration

100 mg/mL in DMSO

solubility

DMSO: soluble

storage temp.

2-8°C

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

InChI key

YPHMISFOHDHNIV-FSZOTQKASA-N

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1 of 4

This Item
C48594640118079
vibrant-m

239765

Cycloheximide

vibrant-m

C4859

Cycloheximide solution

vibrant-m

46401

Cycloheximide

vibrant-m

18079

Cycloheximide solution

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

form

solution

form

solution

form

-

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

DMSO: soluble

solubility

DMSO: soluble

solubility

H2O: slightly soluble

solubility

-

concentration

100 mg/mL in DMSO

concentration

100 mg/mL in DMSO

concentration

-

concentration

0.1%

General description

A convenient ready-to-use form of Cycloheximide (Cat. No. 239763). An anti-fungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Also inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt′s lymphoma cells, and a variety of other cell types, including rodent macrophages. Induces DNA fragmentation in macrophages, but inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.

Biochem/physiol Actions

Primary Target
hFKBP12

Packaging

Packaged under inert gas

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Physical form

A 100 mg/ml solution of Cycloheximide (Cat. No. 239763) in DMSO (sterile-filtered).

Reconstitution

Following initial use, aliquot and refrigerate (4°C).

Other Notes

Christner, C., et al. 1999. J. Med. Chem.42, 3615.
Lu, Q. and Mellgreen,R.L. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Waring, P. 1990. J. Biol. Chem.265, 14476.
Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174.
Pestka, S. 1971. Annu. Rev. Microbiol.25, 487.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3 - Muta. 2 - Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3


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