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InSolution Cycloheximide - CAS 66-81-9 - Calbiochem

InSolution Cycloheximide, CAS 66-81-9, is a 100 mg/ml, sterile-filtered solution of Cycloheximide (Cat. No. 239763) in DMSO. An antifungal antibiotic that inhibits protein synthesis in eukaryotes.

InSolution Cycloheximide - CAS 66-81-9 - Calbiochem
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:

Quality Level




3.4 μM Ki



storage condition

OK to freeze
protect from light

shipped in

wet ice

storage temp.




InChI key


General description

A convenient ready-to-use form of Cycloheximide (Cat. No. 239763). An anti-fungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Also inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt′s lymphoma cells, and a variety of other cell types, including rodent macrophages. Induces DNA fragmentation in macrophages, but inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.


1 ml in Alu drum
Packaged under inert gas

Biochem/physiol Actions

Primary Target


Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Physical form

A 100 mg/ml solution of Cycloheximide (Cat. No. 239763) in DMSO (sterile-filtered).


Following initial use, aliquot and refrigerate (4°C).

Other Notes

Christner, C., et al. 1999. J. Med. Chem.42, 3615.
Lu, Q. and Mellgreen,R.L. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Waring, P. 1990. J. Biol. Chem.265, 14476.
Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174.
Pestka, S. 1971. Annu. Rev. Microbiol.25, 487.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Skull and crossbonesHealth hazard

Signal Word


Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3 - Muta. 2 - Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Certificate of Analysis

Certificate of Origin

Zachary J Waldrip et al.
The Journal of biological chemistry, 297(4), 101209-101209 (2021-09-26)
DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is known primarily for its function in DNA double-stranded break repair and nonhomologous end joining (NHEJ). However, DNA-PKcs also has a critical yet undefined role in immunity impacting both myeloid and lymphoid cell lineages
Joshua D Frenster et al.
The Journal of biological chemistry, 296, 100798-100798 (2021-05-23)
GPR133 (ADGRD1), an adhesion G protein-coupled receptor (GPCR) whose canonical signaling activates GαS-mediated generation of cytosolic cAMP, has been shown to be necessary for the growth of glioblastoma (GBM), a brain malignancy. The extracellular N terminus of GPR133 is thought
Jorge Andrade et al.
Nature cell biology, 23(4), 413-423 (2021-04-03)
Endothelial cells (ECs) adapt their metabolism to enable the growth of new blood vessels, but little is known how ECs regulate metabolism to adopt a quiescent state. Here, we show that the metabolite S-2-hydroxyglutarate (S-2HG) plays a crucial role in

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