251400

D609, Potassium Salt

Selective inhibitor of phosphatidylcholine (PC)-specific phospholipase C (K_i = 5-10 µM).

• Synonym(s): D609, Potassium Salt, Tricyclodecan-9-yl-xanthogenate, K
• Empirical Formula (Hill Notation): C₁₁H₁₅OS₂ · K
• CAS Number: 83373-60-8
• Molecular Weight: 266.46
• MDL number: MFCD00171401

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: off-white
• solubility: DMSO: 25 mg/mL; water: 25 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C11H16OS2.K/c13-11(14)12-10-5-6-4-9(10)8-3-1-2-7(6)8;/h6-10H,1-5H2,(H,13,14);
• InChI key: YFUFHKMDCZBHMY-UHFFFAOYSA-N

Description

General description

Selective inhibitor of phosphatidylcholine (PC)-specific phospholipase C (K_i = 5-10 µM). Does not inhibit phosphatidylinositol-specific phospholipase C, phospholipase A, or phospholipase D. Also reported to inhibit LPS-stimulated ERK activation via its inhibitory effect on PLC. Inhibits angiogenesis by preventing the synthesis of basement membrane.

Selective inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC) (Bacillus cereus, K_i = 5-10 µM). Does not inhibit (up to 50 µM) phosphatidylinositol-specific phospholipase C, phospholipase A₂, and phospholipase D. Inhibitor of the sphingomyelinase activity. Also inhibits angiogenesis by preventing the synthesis of basement membrane (BM).

Biochem/physiol Actions

Cell permeable: no

Primary Target
pc-specificphopholipase c

Product does not compete with ATP.

Reversible: no

Target K_i: 5-10 µM against phosphatidylcholine (PC)-specific phospholipase C

Warning

Toxicity: Standard Handling (A)

Preparation Note

tissue culture media should be at neutral pH, since product is active only at or slightly below pH 7.0. The compound is inactive at pH 7.4 and above, and it is hydrolyzed below pH 6.0. HEPES buffer should not be used as it renders D609 toxic.

Reconstitution

Material is very labile once in solution (1.5 day half-life in tissue culture medium).

Other Notes

Monick, M.M., et al. 1999. J. Immunol. 162, 3005.
Lozanski, G., et al. 1997. Biochem. J.328, 271.
Maragoudakis, M.E., et al. 1993. Kidney Int. 43, 147.
Doppler, C., et al. 1992. AIDS Res. Human Retroviruses8, 245.
Schutze, S., et al. 1992. Cell71, 765.
Sauer, G., et al. 1990. Cancer Lett.53, 97.
Muller-Decker, K. 1989. Biochem. Biophys. Res. Commun.162, 198.
Amtmann, E., et al. 1987. Biochem. Pharmacol.36, 1545.
Sauer, G., et al. 1984. Proc. Natl. Acad. Sci. USA81, 3263.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable