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260913

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DNase I, Bovine Pancreas

Native DNase I from bovine pancreas.

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Synonym(s):
DNase I, Bovine Pancreas, Deoxyribonuclease I
CAS Number:
9003-98-9
Enzyme Commission number:
3.1.21.1 (BRENDA, IUBMB)
MDL number:
MFCD00130918

biological source

bovine pancreas

form

lyophilized

specific activity

≥60,000 dornase units/mg dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

solubility

sodium acetate buffer: soluble
water: soluble

shipped in

ambient

storage temp.

−20°C

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DN25D4263D4513
Millipore

Millipore

260913

DNase I, Bovine Pancreas

Deoxyribonuclease I from bovine pancreas lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein

Sigma-Aldrich

DN25

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas Standardized vial containing 2,000 Kunitz units of DNase I (D4527), vial of ≥0.25 mg total protein

Sigma-Aldrich

D4263

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4513

Deoxyribonuclease I from bovine pancreas

form

lyophilized

form

lyophilized powder

form

powder

form

lyophilized powder

specific activity

≥60,000 dornase units/mg dry wt

specific activity

≥400 Kunitz units/mg protein

specific activity

-

specific activity

≥2,000 units/mg protein

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

-

storage condition

-

solubility

sodium acetate buffer: soluble, water: soluble

solubility

0.15 M NaCl: soluble 5.0 mg/mL, hazy

solubility

0.15 M NaCl: soluble 5.0 mg/mL, clear

solubility

0.15 M NaCl: soluble 5.0 mg/mL, clear(lit.)

General description

Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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