268298

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

• Synonym(s): DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl
• Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2HCl
• CAS Number: 28718-90-3
• Molecular Weight: 350.25
• UNSPSC Code: 12352200
• NACRES: NA.54
• MDL number: MFCD00012681

Properties

• Quality Segment: 100
• description: RTECS - NL5995050
• assay: ≥90% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• color: yellow
• solubility: water: 5 mg/mL, ethanol: soluble, methanol: soluble
• fluorescence: λ_ex ~359 nm, λ_em ~461 nm
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Binds preferentially to DNA rich in adenine and thymine

Product does not compete with ATP.

Reversible: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H317,H335
• pcodes: P261 - P264 - P271 - P272 - P280 - P302 + P352
• Hazard Classifications: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable