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322123

Sigma-Aldrich

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

Sulfhydryl reagent used to characterize reactive SH groups.

Synonym(s):

5,5′-Dithio-bis-(2-nitrobenzoic Acid), DTNB, Ellman’s Reagent

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About This Item

Empirical Formula (Hill Notation):
C14H8N2O8S2
CAS Number:
Molecular Weight:
396.35
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.21

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$144.00


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description

RTECS - DG9650000

Quality Level

assay

≥98% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

ethanol: 8 mg/mL

shipped in

ambient

storage temp.

2-8°C

SMILES string

S(Sc2cc(c(cc2)[N+](=O)[O-])C(=O)O)c1cc(c(cc1)[N+](=O)[O-])C(=O)O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

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This Item
D8130D21820076854
assay

≥98% (titration)

assay

≥98%

assay

99%

assay

≥97.0% (H-NMR)

solubility

ethanol: 8 mg/mL

solubility

-

solubility

-

solubility

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

form

solid

form

powder

form

powder

form

crystals

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

description

RTECS - DG9650000

description

-

description

-

description

-

General description

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Useful in photometric determination of thiols
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Preparation Note

May require gentle heating for complete solubilization.

Other Notes

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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