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324506

Millipore

EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

Synonym(s):

EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated, Ethylenediaminetetraacetic Acid Trisodium Salt

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100 ML
$78.60

About This Item

Empirical Formula (Hill Notation):
C10H13N2Na3O8 · 3H2O
CAS Number:
150-38-9
Molecular Weight:
412.23
MDL number:
MFCD02253006
UNSPSC Code:
12352107
NACRES:
NA.25

$78.60

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Request a Bulk Order

grade

Molecular Biology

Quality Level

100

form

liquid

reaction suitability

reagent type: chelator

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

pH

8

shipped in

ambient

storage temp.

15-25°C

SMILES string

[Na+].[Na+].[Na+].N(CCN(CC(=O)[O-])CC(=O)O)(CC(=O)[O-])CC(=O)[O-].O.O.O

InChI

1S/C10H16N2O8.3Na.3H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;3*1H2/q;3*+1;;;/p-3

InChI key

FXNQQEVEDZAAJM-UHFFFAOYSA-K

Related Categories

General description

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

Application

  • Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification.: This research compares various extraction methods for palaeoproteomic analysis, including those utilizing EDTA, to identify bone species efficiently. The study underscores the importance of EDTA in enhancing protein extraction for proteomic studies (Mylopotamitaki et al., 2023).

  • Deep coverage and quantification of the bone proteome provides enhanced opportunities for new discoveries in skeletal biology and disease.: The use of EDTA in the preparation and extraction of bone samples for proteomic analysis is discussed, emphasizing its role in achieving deep proteomic coverage and facilitating new discoveries in skeletal biology (Rose et al., 2023).

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

hcodes

H318,H373

Hazard Classifications

Eye Dam. 1 - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, Is the DEPC innactivated in this solution ? I have some leftover and I am wondering if it would be suitable for use in a cell culture buffer context ? Thank you in advance for your support, Kind regards, Coline

    1 answer

    1. Yes, DEPC was inactivated. Note that one of the break down products of DEPC is ethanol. DEPC-treated solutions are typically not used in cell culture applications and may be cytotoxic.

      Helpful?

  4. Dear all, Is the EDTA 0.5 M solution prepared with before DEPC treated H2O, or, is it prepared together with DEPC and then autoclaved? Many thanks for your answer! Best, Julia

    1 answer

    1. The solution is prepared with DEPC-treated H2O.

      Helpful?

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