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EGTA, Molecular Biology Grade

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Synonym(s):
EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid
Empirical Formula (Hill Notation):
C14H24N2O10
CAS Number:
Molecular Weight:
380.35
MDL number:

grade

Molecular Biology

Quality Level

assay

≥98% dry basis (complexometric)

form

solid (fine)

reaction suitability

reagent type: chelator

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

1 M NaOH: soluble

cation traces

heavy metals (as Pb): ≤0.001%

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

10-30°C

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

General description

Chelating agent selective for Ca2+. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Application


  • Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).

  • Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).


Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Other Notes

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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