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324682

Sigma-Aldrich

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

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Synonym(s):
Elastase, Porcine Pancreas, High Purity, Crystallized
CAS Number:
Enzyme Commission number:
MDL number:

Quality Level

form

lyophilized

specific activity

≥25 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

solubility

distilled water: 10 mg/mL

foreign activity

Chymotrypsin ≤0.2%

shipped in

ambient

storage temp.

−20°C

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This Item
E0127E0258E7885
specific activity

≥25 units/mg protein

specific activity

≥4.0 units/mg protein

specific activity

≥4.0 units/mg protein (biuret)

specific activity

≥4 units/mg protein

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze, desiccated

storage condition

-

storage condition

-

storage condition

-

solubility

distilled water: 10 mg/mL

solubility

200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear

solubility

-

solubility

-

foreign activity

Chymotrypsin ≤0.2%

foreign activity

-

foreign activity

trypsin ≤50 BAEE units/mg protein

foreign activity

Trypsin ≤50 BAEE units/mg protein

General description

Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.

Physical form

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

Reconstitution

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

Other Notes

Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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