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324699

Sigma-Aldrich

Elastase Substrate IV, Colorimetric - Calbiochem

A specific substrate for pancreatic elastase.

Synonym(s):
Elastase Substrate IV, Colorimetric - Calbiochem
Empirical Formula (Hill Notation):
C25H34N6O9
Molecular Weight:
562.57

Quality Level

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
desiccated

color

white to off-white

solubility

ethanol: 1 mg/mL
water: soluble

shipped in

ambient

storage temp.

−20°C

General description

A specific substrate for pancreatic elastase (km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase, Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.
A specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

Packaging

5 mg in Plastic ampoule

Biochem/physiol Actions

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
Product does not compete with ATP.
Primary Target
A specific substrate for pancreatic elastase
Reversible: no
Cell permeable: no

Warning

Toxicity: Standard Handling (A)

Sequence

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

Physical form

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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