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324708

Sigma-Aldrich

Asp-N Protease

Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain

Synonym(s):

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

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2 μG
$245.00

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2 μG
$245.00

About This Item

CAS Number:
9001-92-7
EC Number:
3.4.24.33 (BRENDA, IUBMB)
MDL number:
MFCD00004464
UNSPSC Code:
12352202
NACRES:
NA.54

$245.00

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Product Name

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi,

grade

Proteomics Grade

Quality Level

100

assay

≥90% (SDS-PAGE)

form

lyophilized solid

specific activity

≥20,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

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Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

324708

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

Endoproteinase Asp-N from Pseudomonas fragi mutant strain suitable for protein sequencing, lyophilized powder

P3303

Endoproteinase Asp-N from Pseudomonas fragi mutant strain

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

324713

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

specific activity

≥20,000 units/mg protein

specific activity

-

specific activity

≥200 units/mg protein

specific activity

≥10 units/mg protein

grade

Proteomics Grade

grade

Proteomics Grade

grade

Proteomics Grade

grade

Proteomics Grade

assay

≥90% (SDS-PAGE)

assay

-

assay

≥90% (SDS-PAGE)

assay

-

form

lyophilized solid

form

lyophilized powder

form

lyophilized

form

lyophilized

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

General description

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Physical form

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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