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Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

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Synonym(s):
Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
CAS Number:
Enzyme Commission number:

grade

Proteomics Grade

Quality Level

assay

≥90% (SDS-PAGE)

form

lyophilized solid

specific activity

≥20,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

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This Item
P3303324715324713
assay

≥90% (SDS-PAGE)

assay

-

assay

≥90% (SDS-PAGE)

assay

-

specific activity

≥20,000 units/mg protein

specific activity

-

specific activity

≥200 units/mg protein

specific activity

≥15 units/mg protein

form

lyophilized solid

form

lyophilized powder

form

lyophilized

form

lyophilized

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

storage condition

OK to freeze

storage condition

-

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze

General description

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Physical form

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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