324831
EGF human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, solid, suitable for cell culture
Synonym(s):
EGF, Human, Recombinant, E. coli, Epidermal Growth Factor, Human, Recombinant, E. coli
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200 μG
$369.00
$369.00
Estimated to ship onMarch 19, 2026Details
About This Item
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
solid
Impurities:
<1.0 EU/μg Endotoxin (EU/μg EGF)
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Product Name
EGF, Human, Recombinant, E. coli,
recombinant
expressed in E. coli
description
Merck USA index - 14, 3527
assay
≥95% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg Endotoxin (EU/μg EGF)
shipped in
ambient
storage temp.
−20°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥97% (SDS-PAGE) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form solid | form lyophilized solid | form liquid | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −70°C |
| shipped in ambient | shipped in ambient | shipped in wet ice | shipped in ambient |
General description
Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to native human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Binding of EGF to its 170 kDa transmembrane receptor results in dimerization of the receptor, activation of the intrinsic receptor tyrosine kinase, and initiation of the complex signal transduction cascade that leads to cell proliferation and/or differentiation. Acts as a survival factor in preventing apoptosis.
Biochem/physiol Actions
ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
Reconstitute to a concentration of ≥10 µg/ml in 10 mM acetic acid containing ≥0.1% HSA or BSA.
Other Notes
Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 2
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