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324870

Sigma-Aldrich

Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

liquid, Calbiochem®

Synonym(s):
Anti-α-ENaC

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SCNN1A(6337)
rat ... Scnn1A(25122)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.
Recognizes the ~97 kDa glycosylated α-ENaC protein.
This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).

Immunogen

Human
a synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein

Packaging

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol.18, 138.
Does not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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