325896

Wee1, GST-Fusion, Human, Recombinant, S. frugiperda

• EC Number: 2.7.1.112 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: liquid

Properties

• form: liquid
• specific activity: ≥1 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C
• Quality Level: 100

Description

General description

Recombinant, human WEE1 fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Katayama, K., et al. 2005. Mol. Cell. Biol.25, 5725.
Yoshida, T., et al. 2004. Ann. Oncol.15, 252.
Watanabe, N., et al. 1995. EMBO J.14, 1878.
Igarashi, M., et al. 1991. Nature353, 80.

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1