341006

Sigma-Aldrich

EDAC, Hydrochloride

EDAC HCl is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis.

Synonym(s):
EDAC, Hydrochloride, EDCI, 1-Ethyl-3-(3ʹ-dimethylaminopropyl)carbodiimide, HCl
Empirical Formula (Hill Notation):
C8H17N3 · xHCl
CAS Number:
Molecular Weight:
155.24 (free base basis)
MDL number:

storage conditions

-20C

Quality Level

assay

≥98% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white

solubility

aqueous buffer: 2-5 mg/mL
water: 2-5 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C8H17N3.ClH/c1-4-11(8-9)7-5-6-10(2)3;/h4-7H2,1-3H3;1H

InChI key

FDXPUDRRFDHONO-UHFFFAOYSA-N

General description

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.
Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.
Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M-1cm-1.

Packaging

5 g in Plastic ampoule
25 g in Glass bottle
Packaged under inert gas

Warning

Toxicity: Irritant (B)

Preparation Note

Avoid amine containing buffers.

Reconstitution

Unstable in solution. Reconstitute just prior to use.

Other Notes

Chazot, P.L., et al. 1993. Biochem. Pharmacol. 45, 605.
Richardson, A., et al. 1992. Biochem. Pharmacol. 43, 1415.
Taniuchi, M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1950.
Chase, J.W., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5480.
Williams, A., et al. 1981. J. Am. Chem. Soc.103, 7090.
Yamada, H., et al. 1981. Biochemistry20, 4836.
Thomas, J.O., et al. 1978. J. Mol. Biol.123, 149.
Ozawa, H. 1970. Biochemistry9, 2158.
Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

hazcat

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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