341006

EDAC, Hydrochloride

EDAC HCl is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis.

• Synonym(s): EDAC, Hydrochloride, EDCI, 1-Ethyl-3-(3ʹ-dimethylaminopropyl)carbodiimide, HCl
• Empirical Formula (Hill Notation): C₈H₁₇N₃ · xHCl
• CAS Number: 25952-53-8
• Molecular Weight: 155.24 (free base basis)
• MDL number: MFCD00012503

Properties

• storage conditions: -20C
• Quality Level: 200
• assay: ≥98% (titration)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: white
• solubility: aqueous buffer: 2-5 mg/mL; water: 2-5 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C8H17N3.ClH/c1-4-11(8-9)7-5-6-10(2)3;/h4-7H2,1-3H3;1H
• InChI key: FDXPUDRRFDHONO-UHFFFAOYSA-N

Description

General description

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.

Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M^-1cm^-1.

Packaging

5 g in Plastic ampoule

25 g in Glass bottle

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Preparation Note

Avoid amine containing buffers.

Reconstitution

Unstable in solution. Reconstitute just prior to use.

Other Notes

Chazot, P.L., et al. 1993. Biochem. Pharmacol. 45, 605.
Richardson, A., et al. 1992. Biochem. Pharmacol. 43, 1415.
Taniuchi, M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1950.
Chase, J.W., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5480.
Williams, A., et al. 1981. J. Am. Chem. Soc.103, 7090.
Yamada, H., et al. 1981. Biochemistry20, 4836.
Thomas, J.O., et al. 1978. J. Mol. Biol.123, 149.
Ozawa, H. 1970. Biochemistry9, 2158.
Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H311 - H315 - H317 - H373 - H410
• Precautionary Statements: P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314
• hazcat: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• storage_class_code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK Germany: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable