341208

Sigma-Aldrich

Exoenzyme C3, Clostridium botulinum

Exoenzyme C3, Clostridium botulinum, CAS 58319-92-9, catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho.

Synonym(s):
Exoenzyme C3, Clostridium botulinum
CAS Number:
MDL number:
Pricing and availability is not currently available.

storage conditions

+2C to +8C

Quality Level

assay

≥90% (SDS-PAGE)

form

solid

mfr. no.

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

General description

Exoenzyme C3, Clostridium botulinum, CAS 58319-92-9, catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho.
Catalyzes the hydrolysis of NAD. Can be used as a reagent for the detection of Rho proteins. Predominantly catalyzes the ADP ribosylation of the Asn41 amino acid residue in Rho. Treatment of cultured cells with exoenzyme C3 has been shown to induce ADP-ribosylation of RhoA protein and to reduce cell growth rate and saturation density. Its growth inhibitory effects appear to result from blockage at the G1 to S phase transition, causing the accumulation of cells in the G1 phase.
Catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho. Inhibits the progression of the cell cycle from G1 to S phase.

Warning

Toxicity: Irritant (B)

Physical form

Lyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5

Reconstitution

Reconstitute with sterile distilled H₂O
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.

Other Notes

Udagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
Yamamoto, M., et al. 1993. Oncogene8, 1449.
Popoff, A., et al. 1991. Infect. Immun.59, 3673.
Morii, N., et al. 1988. J. Biol. Chem.263, 12420.
Hartree, E.F. 1972. Anal. Biochem.48, 422.
Weber, K., and Osborn, M. 1969. J. Biol. Chem.244, 4406.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Risk Statement

37

Safety Statement

36

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