Exoenzyme C3, Clostridium botulinum, CAS 58319-92-9, catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho.
Catalyzes the hydrolysis of NAD. Can be used as a reagent for the detection of Rho proteins. Predominantly catalyzes the ADP ribosylation of the Asn41 amino acid residue in Rho. Treatment of cultured cells with exoenzyme C3 has been shown to induce ADP-ribosylation of RhoA protein and to reduce cell growth rate and saturation density. Its growth inhibitory effects appear to result from blockage at the G1 to S phase transition, causing the accumulation of cells in the G1 phase.
Catalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho. Inhibits the progression of the cell cycle from G1 to S phase.
Toxicity: Irritant (B)
Lyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
Reconstitute with sterile distilled H₂O
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
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